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农杆菌介导的抗寒基因转化羽衣甘蓝的初步研究
作者: 张文玲   来源: 西南大学 年份: 2010 文献类型 : 学位论文 关键词: 抗寒转录调控基因TCF   羽衣甘蓝   甜菜碱合成基因BADH   转基因植株  
描述: siea. Low temperature is the key factor that limits the growth, colour and appreciatability in winter in the northern of China. Introducing foreign gene into kale through genetic engineering may create novel variety with cold tolerance. In this study, BADH(encoding betaine aldehyde dehydrogenase) and TCH(encoding transcription regulation factors) were used to be transformed into kale with Agrobacterium-mediated transformation.
全文:生根培养基MS+0.1mg/LNAA上,根长1cm左右,移栽到营养钵中。 2.建立了农杆菌介导的遗传转化体系 确定了羽衣甘蓝最终的转化条件:取发芽3-4d的无菌苗,切取带柄子叶预培养2d,将二次活化
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